A semi-automated fluorescent (SAF) assay using viable, whole cells for screening hybridoma supernatants
نویسندگان
چکیده
منابع مشابه
A semi-automated fluorescent (SAF) assay using viable, whole cells for screening hybridoma supernatants.
In the production of monoclonal antibodies, a rapid, sensitive, accurate assay is needed for the critical step of screening. We report the modification of an assay using viable whole cells for screening hybridoma supernatants. The modified assay uses fluorescent second antibodies for detection and has been adapted to an instrument capable of automating a number of assay steps. The modified assa...
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ژورنال
عنوان ژورنال: Journal of Immunological Methods
سال: 1987
ISSN: 0022-1759
DOI: 10.1016/0022-1759(87)90220-1